The present invention relates to the method for the decolourization of RGB-Red dye using green alga Chlorella pyrenoidosa in the presence of charcoal. The effect of dye concentrations and pH on the growth and rate of decolourization of chlorella species is analyzed by the use of UV-Visible spectrophotonieter. The use of charcoal in the present method enhances the growth of chlorella pyrenoidosa and also makes the decolourization process fast. The present dye decolourization method suggests the application of green algae C. pyrenoidosa in combination with charcoal as a potent agent to remove RGB-Red synthetic dye from the environment. The present invention relates to a simple and cost-effective dye degradation method of RGB-Red dye using chlorella pyrenoidosa in the presence of charcoal.