The present invention discloses a body fluid collection device, so as to provide a body fluid collection device which is easy to adhere to a human body, preventing peeling off, and capable of collecting a body fluid such as urine or semen. The body fluid collection device of the present invention includes: a fitting part, which is a sheet having an opening and has a first surface and a second surface; an adhesive layer, disposed on the first surface of the fitting part; and a collection film having an edge having a circumference greater than a circumference of the opening of the fitting part and connected to the second surface of the fitting part in a manner of surrounding the opening of the fitting part. The present invention relates to the technical field of body fluid collection device.