nullThe present invention relates to a kind of for preparing or produce the acidic group enzymatic method of edible Nidus collocaliae (EBN) extract,It comprises the following steps: kosher Nidus collocaliae,Add and purify in the EBN that water is the most cleaned,Wherein water is 20:1 to 50:1 with the weight ratio of EBN,Blend described EBN and purify water to form aqueous paste agent solution,At less than 100 DEG C, water proof boils or described " blending " the aqueous paste agent solution that boils was less than 24 hours,Described " blending " the aqueous paste agent solution of cooling is to less than 40 DEG C,Add the acid solution pH to 5 or lower with described " blending " the aqueous paste agent solution of regulation,The enzyme of 0.1% to 5% concentration is added to described " blending " and " acidity " solution mixture to activate acidic hydrolysis process,Enzymatic hydrolysis process at a temperature of less than 75 DEG C continuously less than 24 hours,Raise described solution mixture to higher than 80 DEG C so that enzyme inactivation,The pH of described solution mixture is regulated to neutral with alkaline solution,Filter neutralized solution mixture,Then lyophilization or be spray-dried filtered solution or filtrate to obtain concentrate and high-quality EBN extract.
