Ex-vivo prepared T-cells are harvested from cell culture conditions andformulated in medium suitable for infusion. The formulation is made bylabeling the cells with one or more agents which have reactivity for T-cellsurface moieties capable of delivery activation signals upon cross-linking andmixing the labeled cells with biodegradable nanospheres or microspheres coatedwith a material capable of cross-linking the agents attached to the T-cellsurface moieties. Alternatively, the formulation may be made by mixing apopulation of T-cells with biodegradable nanospheres or microspheres coatedwith a first material and one or more second materials. The first materialbinds the second material and the second material has reactivity for surfacemoieties on the T-cells and the interaction of the second materials with the T-cells causes the activation of the T-cells. In either method, the mixture of T-cells and biodegradable spheres are suspended in a medium suitable forinfusion, and the mixture is packaged in a container.
