University of Pittsburgh-of the Commonwealth System of Higher Education
发明人:
Donnelly, Christopher James,Mann, Jacob R.
申请号：
AU2018231195
公开号：
AU2018231195A1
申请日:
2018.03.07
申请国别(地区):
AU
年份:
2019
代理人:
摘要：
The present disclosure relates to compounds, compositions, and methods for the inducing neurodegenerative disease pathologies. In one aspect, disclosed herein is a nucleotide sequence encoding a chimeric polypeptide, comprising: a first nucleotide sequence encoding a light-induced oligomerization domain and a second nucleotide sequence encoding a neurodegenerative disease target protein. Disclosed herein is a method of inducing a neurodegenerative disease pathology in a cell, comprising the steps: introducing into the cell an expression vector encoding a chimeric polypeptide, comprising: a first nucleotide sequence encoding a light-induced oligomerization domain and a second nucleotide sequence encoding a low complexity domain from a neurodegenerative disease target protein, wherein the first nucleotide sequence is operably linked to a promoter； expressing the chimeric polypeptide； and inducing oligomerization of the chimeric polypeptide by stimulation with blue light.
