The invention concerns an artificial vocal fold (1) consisting of two parts, in the transversal direction the two parts are slidingly placed in opposite walls of guide frame (5) and in the longitudinal direction they are attached to attachment wall (9) of guide frame (5) on one side and to attachment wall (9) of setting frame (6) on the opposite side, whereas inner area (4) of artificial vocal fold (1) is connected to a pressure fluid source.A method of tuning the vocal cord substitution as described in the invention lies in that the basic frequency of the human vocal fold is determined, on the basis of which an initial prestressing of the artificial vocal fold is created by setting-up a distance of guide frames (5) and setting frames (6) enclosing the artificial vocal folds, followed by changing vibration characteristics of the artificial vocal fold through changing the fluid pressure in the artificial vocal fold, the vibration characteristics are monitored and compared to the demanded vibration characteristics of the human vocal fold, until similarity of the compared vibration characteristics of the artificial and human vocal fold is achieved.
