Disclosed herein is an anti-biofilm microstructure including a substrate and a hydrophilic layer. The substrate includes a plurality of pores on the surface of substrate, wherein the plurality of pores extend in the thickness direction of the substrate. The hydrophilic layer is disposed on the surface of the substrate and the walls of the pores. Even if microbial such as bacteria adhere to the anti-biofilm microstructure, it is difficult to form a biofilm.本發明係有關於一種抑制生物膜微結構，其包含一基材，以及一親水層。該基材表面具有複數個孔洞，且孔洞向基材厚度方向延伸，該親水層設置於該基材之表面以及該些孔洞之孔壁。可使細菌等微生物即使附著於該抑制生物膜微結構後，亦不易形成生物膜。
