A device and a method for breeding blueberry-specific mycorrhizal fungi. The device includes a container, where a bottom of the container is laid with perlite to form a perlite layer； an absorbent cotton is arranged on the perlite layer； a hole for planting is provided in the absorbent cotton and blueberry tissue culture seedlings or moss seedlings are placed in the hole； and a spore transfer solution is provided around the blueberry tissue culture seedlings or moss seedlings. When used as a bacterial fertilizer for inoculation in the field, the pieces of absorbent cotton are buried directly around the blueberry roots； while used for research, one of the pieces of the absorbent cotton is washed with sterile water and filtered with a filter paper to obtain mycorrhizal fungi spores.
