The present invention provides a subunit vaccine and nucleotide sequence thereof of Porcine Circovirus Type 2 (PCV2), comprising the combination of coding PCV2 ORF2 DNA fragment Thus, the present invention can generate the specific antibody in order to against PCV2 in swine’s bodies and prevent from the infection of PCV2.本發明係提供一種第二型豬環狀病毒(Porcine Circovirus Type 2, PCV2)之次單位疫苗及其核苷酸序列,其包含組合編碼PCV2 ORF2(Open Reading Frame 2, 開放式讀取框)之DNA片段;藉之,本發明可於豬體內,誘導產生對PCV2之專一性抗體,進而可以保護,可能受PCV2感染之豬隻。