The Regents of the University of Michigan;The Trustees of Columbia University in the City of New York
发明人:
Stojanovic, Milan N.,Narasimhan, Diwahar,Landry, Donald,Sunahara, Roger K.,Brim, Remy L.,Tesmer, John J. G.,Woods, James H.,Macdonald, Joanne
申请号：
AU2008275025
公开号：
AU2008275025B2
申请日:
2008.07.10
申请国别(地区):
AU
年份:
2014
代理人:
摘要：
Provided are compositions comprising a cocaine esterase (CocE) and a compoundthat thermostabilizes the CocE. Also provided are methods of thermostabilizinga cocaine esterase. Additionally provided are methods of treating a mammal undergoinga cocaine-induced condition. Methods of determining whether a compound is athermostabilizing agent for a protein are also provided. Uses of the above-describedcompositions for the treatment of a cocaine-induced condition is additionallyprovided. Additionally provided is an isolated nucleic acid encoding a CocEpolypeptide having the substitutions L169K and G173Q, and the CocE polypeptideencoded by that nucleic acid, and pharmaceutical compositions thereof. Furtherprovided is the use of that composition for the manufacture of a medicament forthe treatment of a cocaine-induced condition and for the treatment of a cocaine-inducedcondition.
