‘UFBest’ is a new and distinct variety of peach tree which has a winter chilling requirement slightly less than 100 chill units. The tree is of large size and is highly vigorous with a semi-spreading growth habit. Flowers are showy and pink and leaf glands are small and reniform in shape. Trees are self fertile and regularly bear heavy annual crops of early season fruit which are large for its ripening season. Fruit are firm, yellow, and non-melting, clingstone flesh. Fruit are uniform, substantially symmetrical shape, attractive, and have a bright red skin over nearly 100% of the deep yellow ground color. The fruit usually ripens 5 days before ‘UFSun’ in late April to early May at Gainesville, Fla.