SET OF SYNTHETIC OLIGONUCLEOTIDES FOR DETERMINATION OF SEQUENCE OF 1 INTRON, ADJACENT TO 2 EXON, GENES OF HLA I AND II CLASS SYSTEM (HLA-A AND HLA-DRB1)
Obshchestvo s ogranichennoj otvetstvennostyu "Nauchno-proizvodstvennaya firma DNK-Tekhnologiya" (OOO "NPF DNK-tekhnologiya")
发明人:
Paramonov Igor Vladimirovich,Trofimov Dmitrij Yurevich,Loginova Mariya Aleksandrovna,Yankevich Tatyana Eduardovna
申请号：
RU20160148236
公开号：
RU2649064(C1)
申请日:
2016.12.08
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: chemistry.SUBSTANCE: invention relates to biochemistry and molecular biology, in particular to a set of synthetic oligonucleotides. Specified set is designed to determine the 1 intron sequence adjacent to the 2 exon of genes of the HLA I and II class system (HLA-A and HLA-DRB1) by the PCR method and consists of the following primers: dr1in6s 5’-GGATCCTCCTCCAGCTCCTG-3’, dr1in2a 5’-CCGCTCCGTCCCATTGAAGA-3’, A1in1s 5’-CTCCGAACCCTCCTCCTGCTA-3’, A1in2a 5’-GCTGTCGAACCGCACGGACTG-3’.EFFECT: invention makes it possible to determine the sequences of the 1 intron adjacent to the 2 exon, the genes of the HLA system of classes I and II.1 cl, 1 dwg
