PROBLEM TO BE SOLVED: To provide a medical kit and a fluid charging method capable of quickly putting the right amount of liquid in each syringe by a simple operation.SOLUTION: A medical kit 100 includes a liquid storage container 5 in which a liquid P is stored in advance, syringes 1a and 1b, a first connector 3 for connecting the liquid storage container 5, and the syringes 1a and 1b, and a regulation mechanism 30 for collectively regulating movement limit of gaskets 7 of the syringes 1a and 1b. In a state that the liquid storage container 5 and the syringes 1a and 1b in an empty state are connected through the syringes 1a and 1b, an operation to send the liquid P in the liquid storage container 5 is performed until the movement limit is regulated so that the liquid P is put in the syringes 1a and 1b collectively.SELECTED DRAWING: Figure 2