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METHOD FOR ENZYMATIC HYDROLYSIS OF HIGH MOLECULAR-WEIGHT NATURAL POLYMER
专利权人:
OVIK LEONARDOVICH MKRTCHYAN
发明人:
OVIK LEONARDOVICH, MKRTCHYAN
申请号:
TR201910522
公开号:
TR201910522T4
申请日:
2014.11.04
申请国别(地区):
TR
年份:
2019
代理人:
摘要:
Usage: the invention relates to biotechnology and medicine, and specifically to methods for the enzymatic hydrolysis of high molecular-weight natural polymers, and can be used in medicine, agriculture, pharmaceutics and cosmetics. Goal: developing a novel method for the enzymatic hydrolysis of a high molecular-weight natural polymer using auto-fermentation while creating optimal conditions for an enzyme-substrate reaction to take place. Essence of the invention: a method for the enzymatic hydrolysis of a high molecular-weight natural polymer, including a preparatory stage involving adding distilled water and heating a homogenate, carrying out hydrolysis while constantly stirring, and judging the quality of a produced hydrolyzate, in which, according to the invention, the high molecular-weight natural polymer is honey, into which 80% purified water is added from the total volume of water to be added, the temperature is brought to 35-40°С and the pH of the homogenate is brought to 6.0, after which, with vigorous stirring and a pH of 6.0, the temperature is increased to 45°С, every ten minutes over the course of 50-60 minutes the pH value is corrected until a stabilized pH value of between 4.5 and 5.0 is reached, the resulting hydrolyzate is cooled to room temperature, stirred, and has ethyl alcohol, in a quantity of 2% of the total volume, added thereto as a stabilizer, the remainder of the water is added, stirred and let sit for 60 minutes, the resulting concentrate of de-enzymed honey is filtered, poured into containers and autoclaved at a temperature of 110°С.Yüksek molekül ağırlıklı bir doğal polimerin enzimatik hidrolizi için, distile su ilavesi ve homojen ısıtmayı içeren bir hazırlık aşamasını, sürekli karıştırma altında hidroliz gerçekleştirilmesini ve bu şekilde üretilen hidrolizatın kalitesinin değerlendirilmesini içeren bir prosestir, buluşa göre, sözü edilen yüksek molekül ağırlıklı doğal polimer olarak bal kullanılmaktadır ve bala, eklenecek toplam su hacm
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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