A heart valve bioprosthesis (100) and a manufacturingmethod thereof are provided. The heart valve bioprosthesis (100) comprises: a valve frame (1), a valve leaflet (2) and a valve auxiliary structure. The valve leaflet (2) is connected to the valve auxiliary structure, the valve auxiliary structure is connected to the valve frame (1). Both the valve leaflet (2) and the valve auxiliary structure are made of biomaterials. The valve frame (1) is integrally formed by an elastic material, and the valve auxiliary structure is preset with a suturing mark. The heart valve bioprosthesis improves the biocompatibility with the human body, reduces the total height of the valve, shortens the suturing time, and decreases the possibility of thrombus or bacterium attachment thereto. In addition, the heart valve bioprosthesis is made by simple processing, has an excellent compliance with the cardiac tissue, and has a prolonged valve service life.
