GOMEZ SEBASTIAN, Silvia,LOPEZ VIDAL, Javier,MARTINEZ ESCRIBANO, José Angel
申请号：
ES12753914
公开号：
ES2611302T3
申请日:
2012.06.12
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
derivative of the genus Lepidoptera insect, comprising nucleic acid elements introduced in the insect by a recombinant baculovirus containing Ac-ie-01 endogenous gene, wherein said nucleic acid elements comprise [1] an additional copy of cDNA Ac -ie-01 baculovirus as transgene under the control of a suitable promoter which allows expression of the IE-1, IE-0 proteins and / or fragments thereof that function as transcription regulators above endogenous levels obtained during baculovirus infection, wherein the nucleic acid is selected from the group consisting of: (a) a nucleic acid containing the nucleotide sequence shown in any of SEQ ID NO: 1- 5 (B) a nucleic acid having a sequence identity of at least 75%, preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90 % and most preferably at least 95% to the nucleotide sequence shown in any of SEQ ID NO: 1-5 and encoding a protein that can function as a regulator of transcription in a recombinant baculovirus (C) a nucleic acid encoding an amino acid containing the amino acid sequence shown in any of SEQ ID NO: 6-9 and (d) a nucleic acid encoding an amino acid sequence having a sequence similarity of at least 70%, preferably at least 75%, more preferably at least 80%, more preferably at least 85% , more preferably at least 90% and most preferably at least 95% with amino acid sequence shown in any of SEQ ID NO: 6-9 and that may function as transcriptional regulator in a recombinant baculovirus [2] in the insect further comprises at least one homologous region (hr) recombinant as region enhancer operably linked to any promoter that is suitable to drive expression of a recombinant protein, [3] wherein the promoter it drives expression of said recombinant protein is selected from the group 35 nucleic acid comprising: i. a nucleic acid containing the nucleotide sequence shown in any of SEQ ID NO: 10- 16 and ii. a nucleic acid that can function as promoter in a recombina
