The present invention relates to an antimicrobial filter adopting optical fibers and to an air cleaner comprising the same. More particularly, the present invention relates to an antimicrobial filter using optical fibers and to an air cleaner comprising the same, in which ultraviolet rays, visible light or natural light is emitted directly through the surface of optical fibers contained in a filtering material, thus effectively killing, in a short time, microorganisms collected in the filtering material, such as bacteria, fungi, or viruses, which are harmful to the human body. Furthermore, a photocatalyst may be coated on the surface of the filtering material to achieve an improved sterilization effect. Thus, the deterioration of the sterilization effect caused by dust particles being continuously collected at the surface of filter fiber, which are disadvantages of existing antimicrobial filters, can be overcome.