AN IMPROVED METHOD FOR RECOVERING THE PROTEIN EXPRESSED BY OPEN READING FRAME 2 FROM PORCINE CIRCOVIRUS TYPE 2 IS PROVIDED. ALSO PROVIDED IS RECOMBINANT PCV2 ORF2 PROTEIN, AND IMMUNOGENIC COMPOSITIONS COMPRISING PCV 2 ORF2 PROTEIN. MOREOVER, MULTIVALENT COMBINATION VACCINES ARE PROVIDED WHICH INCLUDE AN IMMUNOLOGICAL AGENT EFFECTIVE FOR REDUCING THE INCIDENCE OF OR LESSENING THE SEVERITY OF PCV2 INFECTION, PREFERABLY PCV 2 ORF2 PROTEIN, OR AN IMMUNOGENIC COMPOSITION COMPRISING PCV 2 ORF2 PROTEIN, AND AT LEAST ONE IMMUNOGENIC ACTIVE COMPONENT OF ANOTHER DISEASE-CAUSING ORGANISM IN SWINE.