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MICRO-ORGANISMES SECRETANT DE MANIERE EXTRACELLULAIRE DES LIPIDES ET PROCEDES POUR PRODUIRE DES LIPIDES ET DES VESICULES LIPIDIQUES ENCAPSULANT LES LIPIDES UTLISANT LESDITS MICRO-ORGANISMES
专利权人:
SUNTORY LIMITED
发明人:
AKIMOTO, KENGO,KAWASHIMA, HIROSHI,SHIMIZU, SAKAYU
申请号:
CA2347094
公开号:
CA2347094C
申请日:
2000.08.11
申请国别(地区):
CA
年份:
2015
代理人:
摘要:
There are provided microorganisms having a propertyof producing a lipid containing unsaturated fatty acidsas constituent fatty acids and extracellularly secretingthe produced lipid encapsulated in lipid vesicles,methods of screening said microorganisms, as well asmethods of efficiently producing a fatty acid-containinglipid using said microorganisms. Furthermore, there areprovided lipid vesicles encapsulating a lipid containingunsaturated fatty acids, and foods, cosmetics, and animalfeeds comprising said lipid vesicles added thereto.Artificially treated microorganisms ormicroorganisms collected from nature are grown on a solidmedium, and microbial strains that form lipid vesicles atthe periphery of the colonies and/or microbial strainsthat, when cultured in a transparent liquid medium, makethe culture liquid cloudy are selected. Themicroorganisms obtained are cultured, lipid-containinglipid vesicles secreted in the culture liquid, areseparated from the culture liquid, and the lipid isseparated and purified.There are provided microorganisms having a propertyof producing a lipid containing unsaturated fatty acidsas constituent fatty acids and extracellularly secretingthe produced lipid encapsulated in lipid vesicles,methods of screening said microorganisms, as well asmethods of efficiently producing a fatty acid-containinglipid using said microorganisms. Furthermore, there areprovided lipid vesicles encapsulating a lipid containingunsaturated fatty acids, and foods, cosmetics, and animalfeeds comprising said lipid vesicles added thereto.Artificially treated microorganisms ormicroorganisms collected from nature are grown on a solidmedium, and microbial strains that form lipid vesicles atthe periphery of the colonies and/or microbial strainsthat, when cultured in a transparent liquid medium, makethe culture liquid cloudy are selected. Themicroorganisms obtained are cultured, lipid-containing
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