A multiple natural plant extract liquids mixing preparation for alleviating soreness and ache and a manufacturing method thereof are disclosed. The multiple natural plant extract liquids mixing preparation includes an amaranth family extract liquid for 15% of the mixing preparation, a labiate family extract liquid for 20% of the mixing preparation, a first euphorbia family extract liquid for 30% of the mixing preparation, and a first composite family extract liquid for 25% of the mixing preparation. All of the aforementioned extract liquids are mixed to form the mixing preparation. The manufacturing method of the multiple natural plant extract liquids mixing preparation includes the steps of : placing the mixing preparation and grease that are at a ratio of 97:3 into a low-speed stir tank for stirring pouring the stirred mixing liquid into a high-pressure homogeneity emulsifying machine for nanonization and liposome manufactures at the same time. In addition to manufacturing the multiple natural plant extract liquids mixing preparation for alleviating soreness and ache, the mixing preparation with multiple natural plant extract liquids for alleviating ache can be performed with nanonization and liposome manufacture at the same time by adding grease and nanonization process, which is externally applied to the human body for being easily absorbed through skin so as to remedy the damaged cells thereby achieving the function of alleviating soreness and ache of human body.一種緩解酸與痛的多種天然植物萃取液混合製劑及其製法,其多種天然植物萃取液混合製劑包含了:筧科萃取液,占混合製劑比15%;及唇形科萃取液,占混合製劑比20%;及第一大戟科萃取液,占混合製劑比30%;及第一菊科萃取液,占混合製劑比25%;以上全部混合後成為混合製劑。其製法包含了:混合製劑與油脂以97:3之比例置入低速攪拌桶中進行攪拌,攪拌後之混合液注入高壓均質乳化機中,同步完成奈米化及微脂體製作。眾上,除了可以製成緩解酸與痛的多種天然植物萃取液混合製劑外,再加入油脂與奈米化製程,使多種天然植物萃取液混合製劑同步完成奈米化及微脂體製作,經由外部塗抹方式,使人體更易經皮吸收,修復受損的細胞,達成緩解人體酸與痛的功效。