Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of ADPglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of ADPglucose and starch
UNIVERSIDAD PUBLICA DE NAVARRA;CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
发明人:
Miren Edurne Baroja Fernandez,Francisco José Muñoz Perez,Francisco Javier Pozueta Romero,Maria Teresa Moran Zorzano,Nora Alonso Casajus
申请号：
US14267538
公开号：
USRE046642E1
申请日:
2014.05.01
申请国别(地区):
US
年份:
2017
代理人:
摘要：
An isolated sucrose synthase peptide. Also, a method of preparing ADPglucose by incubating the isolated sucrose synthase peptide with ADP in suitable conditions and then isolating and purifying the ADPG produced. Also, an assay kit for the spectrophotometric, fluorimetric or amperometric determination of sucrose, which kit includes the isolated sucrose synthase peptide. Also, a method of producing a transgenic plant that overexpresses sucrose synthase by inserting a genetic construct containing a DNA fragment that encodes the sucrose synthase peptide into a vector and transferring to a plant genome, and a transgenic plant obtained thereby.
