An adjustment system for an Optical Coherence Tomography (OCT) imaging system includes an OCT light source a beam splitter, splitting the OCT light beam into an imaging beam to an imaging arm, and a reference beam to a reference arm a probe, guiding the imaging beam onto a target and receiving a returned imaging beam from the target the beam splitter generating an interference beam from the returned imaging beam and a returned reference beam from the reference arm an imaging detector, detecting the interference beam an imaging processor, generating an OCT image from the detected interference beam and an adjustment device, removably coupled to the probe, the adjustment device comprising the target attached to a distal region of a target holder at a working distance from a distal end of the imaging probe, wherein an optical length of the reference arm is adjustable to improve a calibration of the OCT image.