An access assembly (10) configured to receive one or more surgical instruments is provided. The access assembly (10) includes a compressible port (50) having a proximal end and a distal end, the port (50) defining a plurality of longitudinal passageways (51) extending from the proximal end to the distal end and a flexible cannula assembly (100) received in each of the longitudinal passageways (51) of the port (50). The cannula assemblies (100) each include a seal (142) configured to receive an instrument inserted therethrough in a sealing manner.