Iris Heidmann,Marco Di Berardino,Kim Kok,Grit Schade-Kampmann
申请号：
US14611203
公开号：
US09797855B2
申请日:
2015.01.31
申请国别(地区):
US
年份:
2017
代理人:
摘要：
A method for the determination of pollen viability and/or maturation grade of a pollen population, comprising mechanically removing pollen grains from flowers re-suspending pollen grains in an electrically conductive buffer for keeping the plant cells to remain viable passing the pollen suspension through an appropriate filter with a pore size suitable for a microfluidic device being adapted to perform impedance flow cytometry (IFC) An advantage of the present invention is that a plant cell does not have to be stained for viability analysis, the method is non-invasive and a high number of cells can be analyzed in a short time frame in real-time and on-site. It is possible to follow developmental processes of plant cells, and that it is applicable to all plant species. In particular, the method allows a standardized measurement independent of the location and all over the world.
