artificial antigen-presenting cell, method for expanding tumor infiltrating lymphocytes, treating cancer with a population of tumor infiltrating lymphocytes and for assessing the power of tumor infiltrating lymphocytes, kit, tumor infiltrating lymphocyte population, and, combination
AISHWARYA GOKULDASS,ANAND VEERAPATHRAN,BRIAN RABINOVICH,MICHAEL T. LOTZE
申请号：
BR112019008560
公开号：
BR112019008560A2
申请日:
2017.10.31
申请国别(地区):
BR
年份:
2019
代理人:
摘要：
in some embodiments, compositions and methods related to isolated antigen presenting artificial cells (aapcs) are disclosed, including aapcs comprising a myeloid cell transduced with one or more viral vectors, such as a molm-14 or an em-3 myeloid cell, wherein the endogenously myeloid cell expresses hla-a / b / c, icos-1, and cd58, and wherein the one or more viral vectors comprise a cd86-encoding nucleic acid and a 4-1bbl and / or ox40l-encoding nucleic acid and transduce the myeloid cell to express cd86 and 4-1bbl and / or ox401 proteins. In some embodiments, methods of expanding tumor infiltrating lymphocytes (t115) with aapcs and methods of treating cancers using thyls after expansion with aapcs are also disclosed.em algumas modalidades, as composições e métodos relacionados a células artificiais que apresentam antígeno isoladas (aapcs) são divulgados, incluindo aapcs compreendendo uma célula mieloide transduzida com um ou mais vetores virais, tal como uma molm-14 ou uma célula em-3 mieloide, em que a célula mieloide endogenamente expressa hla-a/b/c, icos-l, e cd58, e em que o um ou mais vetores virais compreendem um ácido nucléico codificando cd86 e um ácido nucléico codificando 4-1bbl e/ou ox40l e transduzem a célula mieloide para expressar cd86 e as proteínas 4-1bbl e/ou ox40l. em algumas modalidades, métodos de expandir linfócitos infiltrantes de tumor (t1l5) com aapcs e métodos de tratar cânceres usando tils depois de expansão com aapcs também são divulgados.
