Arvydas Janulaitis,Remigijus Skirgaila,Dangira Siksniene
申请号：
US15959090
公开号：
US20180298414A1
申请日:
2018.04.20
申请国别(地区):
US
年份:
2018
代理人:
摘要：
A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein； (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex； (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex； (d) subjecting the complexes to reaction conditions which allow target protein activity； and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.
