血管疾患の新たな治療標的を同定するためのインビトロの血行力学的内皮／平滑筋細胞共培養モデルの使用
- 专利权人:
- 发明人:
- 申请号：
- JP20090545600
- 公开号：
- JP5666134(B2)
- 申请日:
- 2008.01.10
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要：
Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis.
- 来源网站：
- 中国工程科技知识中心
