A water supply container for plant cultivation is disclosed, which comprises a water-holding tray, wherein an end of the water-holding tray is configured with a positioning space, and a positioning pillar is formed in upward protrusion from the bottom of the positioning space moreover, the positioning space includes a wall face formed around the periphery of the positioning pillar, in which the wall face corresponding to the middle section of the positioning pillar is configured with a screw thread. In addition, the positioning pillar can be further combined with a water supply bottle fixed to the positioning space of the water-holding tray, and on the outer wall face of the positioning pillar there includes at least a water supply channel so that the liquid flowing down from the water supply bottle enters into the water-holding tray along the water supply channel.
