HUANG SHIHSHIEH;MALVAR THOMAS;LUETHY MICHAEL H;MILLER PHILIP W;GILBERTSON LARRY A;ALLEN EDWARDS;HEISEL SARA E;KOVALIC DAVID K;ROBERTS JAMES K;HOUMARD NANCY;FRIZZI ALESSANDRA;ZHANG YUANJI;GUO LIANG;LUTFIYYA LINDA
发明人:
HUANG SHIHSHIEH,MALVAR THOMAS,LUETHY MICHAEL H.,MILLER PHILIP W.,GILBERTSON LARRY A.,ALLEN EDWARDS,HEISEL SARA E.,KOVALIC DAVID K.,ROBERTS JAMES K.,HOUMARD NANCY,FRIZZI ALESSANDRA,ZHANG YUANJI,GUO LIANG,LUTFIYYA LINDA
申请号：
US20060545082
公开号：
US2007089190(A1)
申请日:
2006.10.10
申请国别(地区):
美国
年份:
2007
代理人:
摘要：
The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
