Disclosed is an antibiotic preparation method, comprising steps as follows: (1) determining targets selected from prokaryotic cells, eukaryotic cells, viruses or products thereof which said antibiotic will react against directly (2) designing a molecular structure of said antibiotic according to the formula F-R: wherein, R is a recognition region, which specifically recognizes or combines said targets F is an effect region, which generates pharmaceutical effects in said targets, and said pharmaceutical effects are effects selected from regulating, repairing, labelling and causing death of said targets specifically comprising making a recognition region molecular structure library making an effect region molecular structure library designing a recombinant molecular structure library using said recognition region molecular structure library and said effect region molecular structure library according to said general formula by structural readjustment, structural recombination and/or structural confirmation of the molecular structures for the recognition region (3) using said recombinant molecular structure library to prepare and verify recombinants to obtain candidate antibiotics (4) screening the candidates from (3) for fulfilment of requirements as a medicine to obtain antibiotics said making a recognition region molecular structure library refers to artificially preparing artificial substances which specifically recognize and/or combine said targets as well as obtaining a molecular structure thereof wherein said artificial substances are antibody mimetics designed according to the amino acid sequence of an immunoglobulin which can specifically recognize a unique substance on said targets.
