A method is described for predicting incipient diabetes, metabolic disorders or the metabolic syndrome by developing a personal temporal Phosphatase profile, which is generated by measuring phosphatase concentration in stool at a single time-point or multiple time-points. The phosphatase profile further can be used for diagnosing and determining prognosis of other incipient or overt diseases, such as the metabolic syndrome, coronary heart disease, nonalcoholic fatty liver disease, cancers, other chronic or acute diseases or infectious diseases. Also described is a specific dose of phosphatase for therapeutic use in incipient diabetes and other incipient or overt diseases.
