Disclosed is a composition for anti-aging, comprising a conditioned cell culture medium obtained by co-culturing umbilical mesenchymal stem cells and oxidative stress damaged cells. Also disclosed is a use of a conditioned cell culture medium in the preparation of a pharmaceutical composition for anti-aging, wherein the conditioned cell culture medium is obtained by co-culturing umbilical mesenchymal stem cells and oxidative stress damaged cells.本發明揭示一種用於抗衰老之組合物,其包含一條件培養基,該條件培養基為臍帶間質幹細胞與氧化壓力損傷的細胞共同培養後的條件培養基。本發明亦揭示一種條件培養基的用途,其用於製備用於抗衰老之醫藥組合物,其中,該條件培養基為臍帶間質幹細胞與氧化壓力損傷的細胞共同培養後的條件培養基。