The present invention relates to a quantitative ex vivo method for the complementary diagnosis of the degree of dental demineralization through the use of Raman spectroscopy, which comprises quantifying the intensities and areas of a plurality of Stoke bands of the spectrum, and defining and calculating indices that result from dividing the areas of certain bands of interest. The diagnosis is determined by the proximity of the values of these indices to the values previously obtained for the normal pattern of dental mineralization established by analyzing healthy dental parts. This method provides a complementary biomedical technique for quantitative ex vivo analysis of the degree of dental demineralization on dental tissue remains extracted by medical prescription, and the information obtained will facilitate the prescription of suitable treatment to reduce or prevent said demineralization.