GORR, Gilbert,ULLISCH, David Alexander,SANKAR-THOMAS, Yantree Devi,SELGE, Thomas,LEIBOLD, Thomas,HECKENMÜLLER, Harald
申请号：
EPEP2016/055989
公开号：
WO2016/150860A1
申请日:
2016.03.18
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
The present invention relates to a method of producing Ingenol, Ingenol esters and/or Tiglian- 3-one derivatives, the method comprising the steps of: (a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives； and (b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a). The present invention further relates to a plant suspension cell culture, wherein the cells are obtained from a plant selected from the family Euphorbiaceae, and wherein the plant cells produce Ingenol and/or one or more Ingenol ester and/or one or more Tiglian-3-one derivatives. The present invention further relates to a plant cell biomass comprising plant cells obtained from the suspension cell culture of the invention, and comprising Ingenol and/or one or more Ingenol esters and/or one or more Tiglian-3-one derivatives. Also, the present invention relates to cryopreserved cells of the plant suspension cell culture of the invention as well as to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives based on these cryopreserved cells.La présente invention concerne un procédé de production d'Ingénol, d'esters d'Ingénol et/ou de dérivés de Tiglian-3-one, le procédé comprenant les étapes consistant à : (a) cultiver des cellules de plante obtenues à partir d'une plante choisie dans la famille des Euphorbiaceae dans un milieu nutritif dans une culture cellulaire en suspension, les cellules produisant de l'ingénol, un ou plusieurs esters d'ingénol et/ou un ou plusieurs dérivés de la tiglian-3-one ； et (b) récupérer l'ingénol, ledit ou lesdits esters d'ingénol et/ou ledit ou lesdits dérivés de la tiglian-3-one produits dans (a). La présente invention concerne en outre une culture cellulaire de plante en suspension, les cellules étan
