The present invention provides anti-ETBR antibody and immunoconjugate and use identical method. In some embodiments, the immunoconjugate including binding the antibody for being covalently attached to a cytotoxic agent ETBR is provided, wherein antibody does not bind an epitope within the amino acid 64 to 101 of SEQ ID: 10.In some embodiments, cytotoxic agent is a Nemorubicin derivative.