Provided are a method of producing retinal pigment epithelial cells, with which the differentiation-induction efficiency of retinal pigment epithelial cells from pluripotent stem cells can be improved and highly pure retinal pigment epithelial cells obtained in a short time through a simple operation a culture method for retinal pigment epithelial cells with which the cells can be stably grown and cultured a toxicity/drug efficacy evaluation method employing retinal pigment epithelial cells useful in transplant therapy and a retinal disease therapeutic drug. The present invention pertains to a method of producing retinal pigment epithelial cells, including a step for adherent culture of human pluripotent stem cells using a culture substrate coated with laminin E8 fragments a culture method for retinal pigment epithelial cells, including a step for adherent culture of retinal pigment epithelial cells using a culture substrate coated with laminin E8 fragments a toxicity/drug efficacy evaluation method employing retinal pigment epithelial cells obtained through production or culture by the aforedescribed methods and a retinal disease therapeutic drug containing the retinal pigment epithelial cells.
