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Manipulation of organic acid biosynthesis and secretion with cirate synthase
专利权人:
AGRICULTURE VICTORIA SERVICES PTY LTD
发明人:
SPANGENBERG, GERMAN,EMMERLING, MICHAEL,ONG, ENG KOK,MENDEZ, RAMIRO,PANTER, STEPHEN,LABANDERA, MARCEL
申请号:
NZ59452204
公开号:
NZ594522A
申请日:
2004.04.14
申请国别(地区):
NZ
年份:
2012
代理人:
摘要:
Disclosed is a substantially purified or isolated nucleic acid or nucleic acid fragment encoding citrate synthase (CS), or complementary or antisense to a sequence encoding CS, and including a nucleotide sequence selected from the group consisting of (a) sequences shown in Figures 4 and 6 hereto (SEQ ID NOS 11 and 13 to 16 respectively); (b) complements of the sequences recited in (a); (c) sequences antisense to the sequences recited in (a) and (b); (d) functionally active fragments and variants having at least approximately 90% identity to the functional part of the sequences recited in (a), (b) and (c) and having a size of at least 30 nucleotides; and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d). Further disclosed is a substantially purified or isolated CS polypeptide from a Lolium species including an amino acid sequence selected from the group consisting of (a) sequence shown in Figure 5 hereto (SEQ ID NO 12); (b) functionally active variants thereof having at least approximately 95% identity to the sequence recited in (a); and (c) functionally active fragments or variants of the sequences recited in (a) from a Lolium species.
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