Brian Edward Oeffinger,Margaret A. Wheatley,Rawan Shraim,Purva Vaidya,John Robert Eisenbrey
申请号：
US16817898
公开号：
US20200289679A1
申请日:
2020.03.13
申请国别(地区):
US
年份:
2020
代理人:
摘要：
One aspect of the invention provides a method for freeze-drying surfactant-stabilized microbubbles. The method includes: preparing vials comprising a mixture comprising microbubbles； partially submerging the vials in a chilled water bath, wherein the water bath has a sub-freezing temperature； placing the vials on a cooled shelf of a lyophilizer； freeze-drying the vials in the lyophilizer； and capping the freeze-dried vials. Another aspect of the invention provides a method for annealing surfactant-stabilized microbubbles. The method includes: preparing vials comprising a mixture comprising microbubbles； passing the vials in and out of liquid nitrogen (LN2) until the mixture is frozen； holding the vials at −；20° C.； placing the vials on a cooled shelf of a lyophilizer； freeze-drying the vials in the lyophilizer； and capping the freeze-dried vials.
