Provided herein, in some embodiments, are methods that include electro stimulating at least one acupoint in a subject, isolating from a blood sample obtained from the subject cells that are immunocytochemically positive for CD 146 (CD146+), and administering to the subject a pharmaceutical composition of cells comprising non-cultured CD146+ stem cells. Also provided herein are pharmaceutical composition and kits comprising the non-cultured CD146+ stem cells.
