Problem to be solved: to provide an insecticide which is low in soil contamination and toxicity and is excellent in insecticidal effect and a method of producing the same.Insecticides containing fatty acid metabolites obtained by metabolizing 4 to 30 fatty acids of 4 to 30 mg / ml in a proteolytic environment under a dissolved oxygen concentration environment of 0.1 to 30 mg and metabolizing 4 to 30 fatty acids into Proteobacteria in a dissolved oxygen concentration environment of 0.1 to 8 mg / L A method of manufacturing an insecticide containing fatty acid metabolites, including a fatty acid metabolic process.土壌汚染や毒性が低く、殺虫効果に優れた殺虫剤、およびその製造方法を提供する。炭素数4~30の脂肪酸を0.1~8mg/lの溶存酸素濃度環境下でプロテオバクテリアに代謝させることで得られる脂肪酸代謝物を含む殺虫剤、および炭素数4~30の脂肪酸を0.1~8mg/lの溶存酸素濃度環境下でプロテオバクテリアに代謝させる脂肪酸代謝工程を含む、脂肪酸代謝物を含む殺虫剤の製造方法。