Obshchestvo s ogranichennoj otvetstvennostyu "Glaukon"
发明人:
Novikov Ivan Aleksandrovich,Subbot Anastasiya Mikhajlovna,Chebotar Igor Viktorovich,Pak Olga Aleksandrovna,Bursov Andrej Igorevich,Sitnikov Andrej Valerevich
申请号：
RU20180108571
公开号：
RU2672356(C1)
申请日:
2018.03.12
申请国别(地区):
俄罗斯
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to biology and medicine and is a method of preparing a biological sample for examination using scanning electron microscopy, including supravital contrasting, characterized in that a two-step contrasting scheme is used, while as the first contrasting agent an aqueous solution is used based on a mixture of magnesium chloride hexahydrate in a concentration of 1.1–1.8 g/l and chloride hexahydrate of a chemical element of the lanthanide group at a concentration of 13.5–20.2 g/l, and an aqueous solution of lead acetate at a concentration of 82–104 g/l is used as the second contrast agent. In one embodiment, the acidity of the solutions is additionally modified using buffer systems based on acetate anion or caustic soda. In one of the options for storing and fixing the sample after contrasting it is treated with a solution of glutaraldehyde or formalin.EFFECT: method provides an increase in the contrast of images of biological objects in backscattered electrons wit
