An improved system for isolating and removing a large mass of tissue from a female patients abdominal cavity during a laparoscopic surgery, includes: (1) a wound retractor platform, (2) a vaginally-insertable, tissue collection bag having a flexible and resilient ring at its open end that is distortable for vaginal insertion but quickly resumes its open and undistorted shaped within the patients abdominal cavity upon insertion, and whose open end receives the large mass of tissue and is then connected to the wound retractor platform so as to form a sealed bag, (3) a tissue cutting instrument which cuts the large mass of tissue into smaller pieces and then aspirates them along with some of the surrounding fluid, and (4) a fluid recirculation system which filters and recirculates the aspirated fluid back through the bag.