According to the present invention, a callus is induced from a ginkgo leaf through a tissue culture, and a culture condition with the excellent antioxidant capacity and maximum growth increment is established. In the result of a tissue culture by using a ginkgo leaf, the NAA addition condition indicates the better callus growth in a light condition and a dark condition in comparison with the 2, 3-D addition condition. The culture condition indicating the best callus growth is 10 &muM NAA/0.5 &muM BA, 10 &muM NAA/0.5 &muM kinetin. Moreover, the antioxidant capacity of the callus extract induced from a ginkgo leaf is the highest when the callus is suspension cultured in a basal MS medium in which 10 &muM NAA is treated in a light condition. In the result of flavonoid glycosides through HPLC, quercetin dehydrate and keamperol is detected as 0.556, 0.157 &mug / 20 &mul in a suspension cultured green callus in a condition in which 10 &muM NAA is treated. The present invention relates to a cosmetic composition containing a callus with the excellent antioxidant capacity. According to the present invention, a callus is induced from a ginkgo leaf through a tissue culture, a culture condition with the excellent antioxidant capacity and maximum growth increment is established, and the cosmetic composition containing the same is provided. In the result of a tissue culture by using a ginkgo leaf, the good callus growth is indicated in a light condition and a dark condition, the best callus growth condition is established, and the cosmetic composition containing the callus with the excellent antioxidant capacity is provided.COPYRIGHT KIPO 2014본 발명에서는 조직배양을 통해 은행나무 잎으로부터 캘러스를 유도하였고, 최대 생장량과 항산화능이 우수한 배양조건을 확립하였다. 즉 은행잎을 이용하여 조직배양한 경과 암과 광조건 모두에서 NAA 첨가 조건이 2,3-D 첨가조건에 비교하여 양호한 캘러스 생장을 나타냈다. 가장 우수한 캘러스 생장을 나타내는 배양조건은 10μM NAA/0.5 μM BA, 10 μM NAA/0.5 μM kinetin으로 나타냈다. 또한 은행잎 유래 캘러스 추출물의 항산화능은 광조건에서 10 μM NAA가 처리된 기본 MS배지에서 캘러스를 현탁 배양하였을 때 가장 높게 나타났다. HPLC를 통한 flavonoid glycosides를
