A kind of seedling sterile culture device of what is closed in this creation system,Its culture apparatus mainly includes culture vessel and adhesive tape; The culture vessel is the container of plastic cement integrated molding,And be that arrangement is recessed with the opening up accommodating space of plural number,It is to be formed with isolation part between each accommodating space,So that each accommodating space is a separate space,The seedling that each accommodating space is cultivated will not mutually pollute completely,And then improve seedling survival rate; The culture medium of process sterilization Hou or culture solution packing are fills up to the culture vessel sterilized and are implanted into seedling Hou,It is that direct what culture vessel open upper end is pasted and pastes adhesive tape,It is all sealed with the opening of the accommodating space by plural number upward,Not only operation procedure is simple and convenient,It more can provide abundant light required for seedling,And can effectively block external microbe or bacterium enters in culture vessel,So that reach free of contamination best culture environment in culture vessel,The effect of improving seedling yield and uniform Fast Growth.本創作係關於一種種苗無菌培養裝置,其培養裝置主要包括有培養容器以及透明膠帶;該培養容器為塑膠一體成型之容器,且係排列凹設有複數開口朝上之容置空間,每一容置空間之間係形成有隔絕部,俾使每一容置空間為一獨立空間,該每一容置空間所培育之種苗完全不會相互汙染,進而提高種苗存活率;該經過殺菌後的培養基或培養液分裝充填至已殺菌的培養容器並植入種苗後,係直接於培養容器開口上端黏貼貼覆透明膠帶,以將複數朝上之容置空間的開口全部加以密封,不但作業程序簡單方便,更可提供種苗所需要的充分光線,且能有效阻絕外界微生物或細菌進入培養容器內,俾使培養容器內達到無汙染的最佳培養環境,提高種苗產量及均勻快速成長之功效。