De Frees, Shawn,Zopf, David,Bayer, Robert,Bowe, Caryn,Hakes, David,Chen, Xi
申请号：
ES10012798
公开号：
ES2561985T3
申请日:
2002.10.09
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
A method of forming a conjugate between an antibody peptide and a modifying group, wherein said modifying group is covalently linked to said antibody peptide by means of an intact glycosyl linker group, said antibody peptide comprising a residue of glycosyl having the formula: ** Formula ** in which a, b, c, i, j, k, l, q, r, s, t, u and z are independently selected members of 0 and 1； m is independently selected from 0 and 1, or m is independently selected from 0-20, when said antibody peptide is anti-HER2 antibody peptide； e, f, g and h are members independently selected from the whole numbers 0 to 4； n, v, w, x e y are 0； R is a modifying group, a mannose or an oligomanose； and R 'is a member selected from hydrogen and a glycosyl residue, a glycoconjugate, and a modifying group, and wherein, when said antibody peptide comprising a glycosyl residue is an anti-RSV F peptide, it has the formula : ** Formula ** in which a-z, R and R 'are as defined above and p is independently selected from 0 and 1； said method comprising: (a) contacting said antibody peptide with a glycosyltransferase and a modified glycosyl donor, comprising a glycosyl moiety that is a substrate for said glycosyltransferase bonded by covalently linked to said modifying group, under appropriate conditions for the formation of said intact glycosyl linker group, wherein said modifying group is poly (ethylene glycol), preferably linear or branched poly (ethylene glycol), preferably a poly (ethylene glycol) having a homodispersed molecular weight distribution.Un procedimiento de formación de un conjugado entre un péptido de anticuerpo y un grupo modificador, en el que dicho grupo modificador está unido mediante enlace covalente a dicho péptido de anticuerpo por medio de un grupo ligador glicosilo intacto, comprendiendo dicho péptido de anticuerpo un residuo de glicosilo que tiene la fórmula:**Fórmula** en la que a, b, c, i, j, k, l, q, r, s, t, u y z son miembros seleccionados de manera independiente de
