A patient-specific navigational guide (1, 1′) for use in spinal surgery, comprises two tubular guiding member (2) extending from a proximal opening (2a) and a distal opening (2b) for guiding a surgical operation on a patient's vertebra (100, 100′). The two tubular guiding members are integral with a bearing frame (3) comprising at least five contact members (11, 15, 16) designed to match with a corresponding plurality of contact areas on the patient's vertebra (100, 100′) in order to define a unique coupling configuration of the patient-specific navigational guide (1, 1′) on the patient's vertebra (100, 100′). The contact members (11, 15, 16) comprise a main contact member (11), designed to couple with a main contact area corresponding to the spinous process (101) of the patient's vertebra (100, 100′) in said coupling configuration, and at least one pair of first and second auxiliary contact members (15, 16), designed to abut on auxiliary contact areas, respectively corresponding to the laminae (102) of the patient's vertebra (100) and to the facet (103) or to the transverse processes (104), in said coupling configuration. The bearing frame (3) comprises a V-shaped bridge (5), connecting the two tubular guiding members (2) and a non-rectilinear bridge (8) further connecting the two tubular guiding members (2) at proximal portions of the tubular guiding members (2), near the proximal openings (2a), and comprises at least one summit portion (8a) defining the more proximal portion of the navigational guide (1).La présente invention concerne un guide de navigation spécifique à un patient (1) destiné à être utilisé en chirurgie rachidienne, comprenant deux éléments de guidage tubulaires (2) d'un seul tenant avec un châssis de support (3) et s'étendant d'une ouverture proximale (2a) à une ouverture distale (2b) pour guider une opération chirurgicale sur une vertèbre d'un patient (100) ; des éléments de contact (14, 15, 16) conçus pour correspondre à une pluralité correspo