The present disclosure relates to an isolated chimeric antigen receptor and modified T cell containing same and use thereof. Particularly, the present disclosure relates to CD19 chimeric antigen receptor, the method for knocking out TARC, B2M and PD-1 gene in T cell in vitro based on CRISPR-Cas9 system, a crRNA used in this method and a T cell with gene knocking out obtained according to the method and use thereof.本公開涉及一種分離的嵌合抗原受體以及包含其的修飾T細胞及用途。具體地，本公開涉及結合CD19的嵌合抗原受體，基於CRISPR-Cas9系統體外剔除T細胞中TARC、B2M和PD-1基因的方法，該方法中使用的crRNA以及根據本公開的方法獲得的基因剔除的T細胞及其用途。
