Disclosed is an application of harmine derivatives in the preparation of antimicrobial agents, wherein the bacteria are selected from Acinetobacter, Bacillus, Campylobacter, Chlamydia, Chlamydia trachomatis, Clostridium, Citrobacter, Escherichia, Enterobacteriaceae, Enterococcus, Francis Escherichia, Haemophilus, Helicobacter, Klebsiella, Listeria, Moraxella, Mycobacteria, Neisseria, Proteus, Pseudomonas, Salmonella, Serratia, Shigella, Stenotrophomonas, Staphylococcus, Streptococcus or Yersinia.La présente invention concerne une application de dérivés dharmine dans la préparation dagents antimicrobiens, les bactéries étant sélectionnées parmi Acinetobacter, Bacillus, Campylobacter, Chlamydia, Chlamydia trachomatis, Clostridium, Citrobacter, Escherichia, Enterobacteriaceae, Enterococcus, Francis Escherichia, Haemophilus, Helicobacter, Klebsiella, Listeria, Moraxella, Mycobacteria, Neisseria, Proteus, Pseudomonas, Salmonella, Serratia, Shigella, Stenotrophomonas, Staphylococcus, Streptococcus ou Yersinia.