The present invention relates to the preparation method of bottle and system.Some embodiments are related to the application of device, such as freeze-drying apparatus, to carry out this method.Exemplary bottle preparation method includes：Multiple bottles are placed in temperature controlled environment；Wherein, each of multiple bottles has the volume of material wherein and each defining therein unfilled volume, each bottle has the plug of the partial insertion bottle opening, therefore gas can be shifted between unfilled volume and external volume；To environment applying vacuum, the pressure in the unfilled volume of environment and each bottle is reduced to first pressure level；To environmental emission inert gas, the pressure in the unfilled volume of environment and each bottle is improved to second pressure level；Under second pressure level, bottle is set to stand the predetermined time in the environment；At least it is repeated once application, discharge and stands；And after repetition, by the fully-inserted each opening of plug to seal each bottle.
