The present invention provides methods to promote the differentiation ofpluripotent stem cells and the productsrelated to or resulting from such methods. In particular, the presentinvention provides an improved method for the formation of pan-creatichormone expressing cells and pancreatic hormone secreting cells. In addition,the present invention also provides methods topromote the differentiation of pluripotent stem cells without the use of afeeder cell layer and the products related to or resulting fromsuch methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producingcells derived from pluripotent stem cells.
